Izon's most popular qEV column is now available in a new proprietary, agarose resin: introducing the qEVoriginal / 35 nm Gen 2.
qEVoriginal Gen 2 columns separate particles using size-exclusion chromatography. The qEVoriginal / 35 nm Gen 2 column has an optimal recovery range of 35nm to 350 nm.
Selecting the most optimal column size for your sample is important for maximising the purity of EVs in your sample. The qEVoriginal Gen 2 column dimensions and resin volume is optimised for 500 μL sample loading.
Both the 35 nm and 70 nm qEVoriginal Gen 2 columns uses the new agarose resin, Gen 2, which provides better separation than resin used in our existing Legacy columns. qEVoriginal columns are now available in both Gen 2 and Legacy resins.
The qEVoriginal Gen 2 is compatible with the Automatic Fraction Collector (AFC) with the qEVoriginal column mount. You can use the AFC to adjust the purified collection volume (PCV) to suit your your needs.
All of Izon's qEV columns are certified to ISO 13485 (2016).
Product Code: ICO-35
Videos
Files
| Attachment | Size |
|---|---|
 qEVoriginal Gen 2 Quick Start Guide | 214.66 KB |
| qEVoriginal Gen 2 user Manual | 389.14 KB |
| qEV columns recommendation | 680.87 KB |
| qEV overview Brochure | 2.67 MB |
| Isolating EVs from Urine using qEV | 165.11 KB |
| Isolating EVs from Blood Products using qEV Columns | 339.18 KB |
| Isolating EVs from cell culture media using qEV | 315.71 KB |
| How to Store Extracellular Vesicles: A Comprehensive Guide on EV Storage Across a Variety of Samples | 1.68 MB |
- Shorter operating time: qEV are significantly faster than ultracentrifugation-based isolation methods
- Improved sample quality: removal of protein and lipoprotein contaminants, as well as particulate ones. Greatly reduced risk of protein complex formation and vesicle aggregation.
- Standardised operation: removes operator bias and dramatically improves consistency in vesicle yield vs both ultracentrifugation and precipitation methods, which under and over-represent yield respectively.
- Non-destructive: qEV is a gentle preparation method in comparison to both ultracentrifugation and precipitation methods and maintains the integrity and biological function of your sample.
- Compatibility with isotonic buffers: ensures that vesicles stay intact. In comparison, with density centrifugation and other methods sugar is added, which destroys vesicles.
- Increases Sensistivity of Analytical Methods. Greater than 50% recovery of vesicles, with high purity means greatly improves the senstivity and usefulness of analytical methods for measuring EVs
- Rapid, Low cost and convenient. qEVs have a low cost-per test, with individual columns re-usable for up to five tests. The ability to perform multiple tests in parallel offers scalability to your experiments.
- Simple. Don’t spend time learning protocols. Unlike other separation techniques qEV’s operation is straightforward and can be learned in 1 minute.
Technical Features
Comparison with other Techniques
| Method | Purity | Exosome yield | Protein Yield | Ease of use | Turn around Time (hrs) | Hands On time (hrs) | Cost per test (USD) |
| UC | ** | *** | ** | ** | 4 | <1 | 6.25 |
| ODG | **** | **** | * | * | 20 | 1 | 18.75 |
| EQ | * | * | *** | *** | 13 | <0.5 | 18.75 |
| TEI | * | * | **** | *** | 13 | <0.5 | 6.25 |
| qEV | **** | **** | * | * | <0.5 | <0.5 | 13 [1] |
| Legend *=low, **=moderate, ***=high ****=very high | |||||||
[1] Dependant on reuse